Services
Basic High-Throughput Sequencing Analysis
Pipelines for analysis should be specifically defined to meet the particular needs of your project but in general it can include:
- QC analysis of fastq files
- Alignment to genome
- QC analysis of aligned reads
- Standard data processing pipelines (eg. GATK)
- Variant Calling, for exome or targetted panels
- Differential gene expression analysis, for RNA-Seq
- Identification of novel splice variants, for RNA-Seq
- 10x single cell cellranger pipeline (gene expression, immune profiling, ATAC, …)
- Methyl-seq pipeline
- ATAC-seq pipeline
- HiC-Pro pipeline
Advanced Data Analysis
Advanced high-throughput sequencing data analysis is also quoted on a per project basis. Advanced data analysis can include:
- Gene ontology
- Pathway analysis
- Single cell data analysis (single cell gene expression, Immune profiling, multiome ATAC + gene expression, CITE-seq, etc)
- Methyl-seq data analysis
- Spatial transcriptomics
- Consistently expanding our workflows to offer a diverse range of data analysis services while incorporating customer feedback.
High Performance Computing (ONLY available for UHN researchers)
Our group manage UHN HPC clusters. Our clusters contain thousands of cores and several PB storage. Researchers are processing data and performing complex calculations every day on the clusters such as NGS data analysis and machine learning. We also manage software stacks and data sets on the clusters. If you want to use HPC services or have issues on the cluster, please contact hpc_support@uhn.ca. Please note, the HPC service is for UHN researchers only.